Journal: Scientific Reports

Article Title: CD206 + tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

doi: 10.1038/s41598-019-51149-1

Figure Lengend Snippet: Association of tumor-associated macrophages (TAMs) with clinicopathologic characteristics in OSCC.

Article Snippet: After rinsing, the cells were incubated at normal temperature for 20 min in dark with PE anti-human CD163 antibodies (Clone GHI/61, IgG1, κ ; BioLegend), FITC anti-human CD204 antibodies (Clone REA460, IgG1; Miltenyi Biotec, Bergisch, Gladbach, Germany), and PerCP/Cy5.5 anti-human CD206 antibodies (Clone 15-2, IgG1, κ ; BioLegend).

Techniques: Significance Assay

Journal: Scientific Reports

Article Title: CD206 + tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

doi: 10.1038/s41598-019-51149-1

Figure Lengend Snippet: Distribution of tumor-associated macrophages (TAMs) and epithelial growth factor (EGF) in oral squamous cell carcinoma (OSCC) patients. ( A ) Representative images of paraffin sections in/around tumors stained with H&E (a–c) and CD163 (d–f), CD204 (g–i), CD206 (j–l), and EGF (m–o) antibodies (brown). Counterstaining with Mayer’s hematoxylin is shown in blue. Scale bars, 100 μm. ( B ) Triple immunofluorescence staining was performed with CD163 (green), CD204 (red), and CD206 (white); nuclei were stained with DAPI (blue). Scale bars, 50 μm. ( C ) Double immunofluorescence staining performed with EGF (red) and CD163, CD204 or CD206 (green); nuclei were stained with DAPI (blue). Merged TAM markers and EGF images (yellow). The higher magnifications are displayed at the lower right. Scale bars, 50 μm.

Article Snippet: After rinsing, the cells were incubated at normal temperature for 20 min in dark with PE anti-human CD163 antibodies (Clone GHI/61, IgG1, κ ; BioLegend), FITC anti-human CD204 antibodies (Clone REA460, IgG1; Miltenyi Biotec, Bergisch, Gladbach, Germany), and PerCP/Cy5.5 anti-human CD206 antibodies (Clone 15-2, IgG1, κ ; BioLegend).

Techniques: Staining, Immunofluorescence, Double Immunofluorescence Staining

Journal: Scientific Reports

Article Title: CD206 + tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

doi: 10.1038/s41598-019-51149-1

Figure Lengend Snippet: Survival curves according to the expression of TAM subsets in OSCC. Survival rates were calculated by the Kaplan-Meier method with high versus low expression of CD163 + , CD204 + , or CD206 + TAMs. The classifications are described in the Materials and Methods section. Statistically significant differences between groups were determined by log-rank test ( A ) and ROC curve ( B ).

Article Snippet: After rinsing, the cells were incubated at normal temperature for 20 min in dark with PE anti-human CD163 antibodies (Clone GHI/61, IgG1, κ ; BioLegend), FITC anti-human CD204 antibodies (Clone REA460, IgG1; Miltenyi Biotec, Bergisch, Gladbach, Germany), and PerCP/Cy5.5 anti-human CD206 antibodies (Clone 15-2, IgG1, κ ; BioLegend).

Techniques: Expressing

Journal: Scientific Reports

Article Title: CD206 + tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

doi: 10.1038/s41598-019-51149-1

Figure Lengend Snippet: Univariate analysis of progression-free survival and disease-specific survival in advanced stage.

Article Snippet: After rinsing, the cells were incubated at normal temperature for 20 min in dark with PE anti-human CD163 antibodies (Clone GHI/61, IgG1, κ ; BioLegend), FITC anti-human CD204 antibodies (Clone REA460, IgG1; Miltenyi Biotec, Bergisch, Gladbach, Germany), and PerCP/Cy5.5 anti-human CD206 antibodies (Clone 15-2, IgG1, κ ; BioLegend).

Techniques: